Methods The levels of lipoprotein in serum of posthepatitic cirrhosis and control grope were detected by using enzyme analysis and immune turbidimetric analysis. 方法采用直接酶法和免疫透射比浊法检测肝硬化患者和正常对照组血清脂蛋白水平。
Diagnosing septicemia should depend on bacteria culture, plasmid analysis restriction enzyme analysis of plasmid DNA, oMP determination and combining medical history and clinical manifestation. 确诊败血症必须通过细菌培养、质粒分析、质粒DNA限制酶分析及OMP测定并结合病史、临床表现。
The DNA vaccine was identified by restriction enzyme analysis, sequencing and expression. 对此疫苗进行酶切、测序和表达鉴定。
Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA 犬Ⅰ型腺病毒DNA的酶切分析及分子克隆
And the results were further confirmed by restriction enzyme analysis of the genomic DNA fragments. 限制性酶切分析其基因组DNA。
Recent applications of ECL in bioanalysis are reviewed, which includes applications of ECL in biosensor, immuno sensor, DNA probe, surface analysis and enzyme analysis. 内容涉及电化学发光在生物传感器研究、免疫分析和DNA探针、表面分析和酶分析中的应用。
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restriction enzyme analysis show that both genes are well-constructed suitable for transgenic animal experiment. 限制性酶切图谱分析证实这两个基因的结构是完整的,符合转基因实验要求。IBL对碱敏感。
Subcloning and comparative restriction enzyme analysis were carried out with the replication origin from tne integrated F 'plasmid and that from the autonomous F plasmid. No structural difference was found between them. 对这一复制起点和来自自主状态的F质粒的复制起点进行了亚克隆,并作限制性内切酶酶切分析比较,没有发现两者在结构上有差异。
Micromorphological Characteristics and Enzyme Analysis of Some Chinese Pinellias and Their Taxonomic Significance 部分国产半夏属植物的微形态特征和同工酶分析及其分类学意义
By blue white screening, the plasmid DNA extracted from white colony was identified using restriction enzyme analysis and the DNA sequence was determined following recon determination. 对PCR扩增的目的片段用1%琼脂糖凝胶电泳检测后,进行回收、连续转化,通过蓝白筛选挑取白色菌落提取质粒DNA进行酶切鉴定,确定重组子后采用双脱氧终止法测定DNA全序列。
Methods: By using microsomal enzyme analysis technique, determine the transforming rate of substrate cortisol by two enzymes after adding glycyrrhizic acid etc, and compare their difference. 方法:运用微粒体酶分析技术,测定加入甘草酸等化合物后,两种酶对底物皮质醇的转化率,并比较两者的差异。
Retro with DNA recombinant technique, and the recombinant vector was confirmed by the restrictive enzyme analysis and DNA sequencing. retro,并用限制性内切酶酶切和测序鉴定;
Polymerase chain reaction and restriction enzyme analysis of human cytomegalovirus infection in pregnant women and neonates 孕妇及新生儿巨细胞病毒感染的聚合酶链反应及限制酶分析
Methods DNA samples from 22 unrelated patients ( 21 male and 1 female) with G6PD deficiency from Tianjin area in North China were studied for the three common mutations by using both mismatched primers mediated polymerase chain reaction/ restriction enzyme analysis and dideoxy fingerprinting methods. 方法采用错配引物介导的聚合酶链反应/限制性酶切分析法和双脱氧核苷酸指纹印迹检测法,对天津地区22例G6PD缺陷患者进行3种中国南方人群常见的G6PD基因突变的分析。
In this study, combining traditional S1 enzyme analysis with sandwich hybridization, sandwich hybridization integrated with nuclease protection assay ( NPA-SH) was developed. 本研究利用S1酶能够保护完全匹配的双链核酸的性质,将分子生物学中的酶保护分析技术和夹心杂交技术融合在一起,创立了双特异分子探针技术以实现对赤潮藻的检测。
Determination of Glucose Content in Milk by Enzyme Analysis 奶制品葡萄糖含量的酶法分析
Restriction enzyme analysis of human papilloma virus ( hpv) dna from common warts 人乳头瘤病毒DNA的限制性核酸内切酶分析
Methods Activity of F ⅴ was determined by biochemical method. The PCR products of F ⅴ gene was analysed by directly sequencing or sequencing after cloned into T vector. The mutative F ⅴ gene was analysed by restriction enzyme analysis in the proband and her family members. 方法利用发色底物法检测血浆FⅤ凝血活性,采用PCR产物直接测序或TA克隆后测序、限制性酶切分析先证者FⅤ基因,并进行家系和FⅤ基因多态性频率研究。
Results The structure of the adenovirus vectors carrying GM-CSF gene and GFP gene was identified by restriction enzyme analysis. The titer of the virus was 7 × 10~ 10 pfu/ ml. 结果经过酶切鉴定成功地构建了分别携带GM-CSF基因和GFP报告基因的腺病毒载体,病毒滴度为7×1010pfu/ml。
The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restriction enzyme analysis. PCR检测和酶切鉴定结果表明,在水平测试的混合饲料粉中可检出牛源、羊源性成分。
The study on restriction endonuclease enzyme analysis method in atypical pneumonia 传染性非典型肺炎限制性内切酶检测方法的研究
Identification of 12 Mycobacteria to the Species Level by Polymerase Chain Reaction and Restriction Enzyme Analysis 采用PCR法和限制性内切酶分析对12种分枝杆菌进行鉴定
These two fragments were cloned into the pMD-18T vector for the partial sequencing and restriction enzyme analysis. 将扩增产物克隆到pMD-18T上,经酶切分析、部分序列测定和同源比较确认得到了所需的基因片段。
Rapid Preparation and Restriction Enzyme Analysis of the DNA of Canine Adenovirus Type 2 犬Ⅱ型腺病毒DNA的快速提取及限制性酶切分析
The recombinant plasmid of pGTE-TK was identified by restriction enzyme analysis and sequencing, which proved completely its validity. 对重组质粒进行限制性内切酶分析和基因测序,证实了克隆片段的可靠性。
Restriction enzyme analysis confirmed: the proband is the missense mutation carrier, MH susceptible person. 限制酶分析确认:临床确诊者及其母亲为该错义突变携带者,为MH易感者。
Making full use of scientific theories and methods, such as Natural products chemistry, Tea-processing, Tea-biochemistry, Enzymology, Computer-aided design and so on, then drawing on the new research system of high-throughput screening and enzyme analysis to carry on the study. 在研究中充分运用天然产物化学、制茶学、茶叶生物化学、酶学和计算机辅助设计等学科理论知识和方法,借鉴高通量筛选、酶学分析法等新的研究手段系统开展工作。
Therefore, the one-step enzyme analysis method proposed in this thesis combined with WE system to perform multi-dimensional screening is worth of in-depth research and further development. 基于此理念,将一步酶分析技术与大管电泳体系相结合并实现多维、高通量筛选平台的初步设想是值得探索和深入研究的。
Enzyme is a protein which has catalytic activity and the enzyme analysis has great influence in the field of life science research. 酶是由细胞产生的具有催化功能的蛋白质,酶分析在生命科学研究中具有重要的意义,已深刻影响到生物、化学等领域。